Part:BBa_K1840008:Design

Device: TT-gad-mCherry

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 325
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Considerations were made regarding the actual start of the promotor. As promotor sequence we assumed all DNA between the gad gene and the previous gene (upstream). Since this gene is encoded on the opposite strand, the terminator was needed.

Source

The double terminator was already registered in the iGEM catalogue. The kgu promotor comes from the genome of Pseudomonas putida. The mCherry is codon optimized for expression in Pseudomonas. The whole device was synthesized by IDT.